The Brandt lab is proud to be a member of the Vision Research Core, and provides Gene Delivery services. If you are interested in core services, please access the core link or contact Dr. Brandt.
Consulting – Gene Delivery Strategies
Consultation is provided regarding the best type of vector system for each intended use. Consideration is given to the size of the expression construct, the level of expression desired, the length of time that expression is needed, potential use of tissue or cell-type specific promoters, the titers of vector needed, the potential for negative effects of the vectors (e.g. inflammation), and whether integration of the transgene is desirable.
Vector construction and high-titer vector stock preparation
Core clients are assisted with construction of the desired vector or, alternatively, the Gene Delivery Core will construct the vector for you. Some clients have expressed the desire to learn how to construct vectors themselves, or more importantly, to have a graduate student or post-doctoral fellow learn this valuable skill. In some instances, we will work side by side with these individuals to train them in the needed skills.
In conjunction with the Pathology and Imaging Core, we can provide assistance with the preparation of primary cultures of various ocular tissues. Investigators may have the need to test their vectors for expression and other properties in the specific cell types they are targeting in vivo. To date, we have provided primary trabecular meshwork cells, primary human corneal keratocytes, and primary ciliary muscle cells for clients.